Modified sublimation to isolate phenanthrene-degrading bacteria of the genera Sphingomonas and Burkholderia from Xiamen oil port     

X. Huang  Y. Tian  Y.R. Luo  H.J. Liu  Wei Zheng  T.L. Zheng   《Marine pollution bulletin》2008,57(6-12):538

Sublimation was developed by Alley and Brown (2000) in order to isolate bacterial strains that were capable of degrading water insoluble compounds. In this study, sublimation was modified by the use of nutritional agar plates, instead of mineral salt agar, to isolate phenanthrene-degrading bacteria from a mixed culture that had been enriched under the selective pressure of high phenanthrene content. Five strains were obtained with different morphology and degradation ability. Based on the 16S rDNA sequence, two of them were classified as species of the genus Sphingomonas; the others as species of the genus Burkholderia. Denaturing gradient gel electrophoresis (DGGE) was introduced to detect dynamic changes in the bacterial community during enrichment batch culture, and to determine any correlation between the five isolates and the phenanthrene-degrading consortium. The DGGE profile indicated that these five isolates corresponded to four dominant bands of the consortium. Compared to traditional means of isolation, we concluded that modified sublimation is effective and more convenient.  相似文献   

InSAR在云南德钦县一中河泥石流物源汇集及失稳模式中的探究     

陈庭轩  李素敏  袁利伟 《大地测量与地球动力学》2022,42(2):158-164

针对传统方法难以定量描述大尺度泥石流形成机理的问题,提出一种联合SAR卫星影像相位信息和后向散射信息全面识别泥石流发育机制的方法,并使用该方法探究云南省德钦县一中河泥石流物源汇集及失稳模式.通过后向散射系数反演研究区土壤含水量的时空变化趋势,利用升降轨Sentinel-1 A数据获取坡表二维形变.结果表明,研究区土壤含...  相似文献   

Growth histories derived from otolith microstructure of three Norwegian cod stocks co-reared in mesocosms; effect of initial size and prey size changes   总被引：2，自引：1，他引：1  

Suthers  I. M.; Van Der Meeren  T.; Jorstad  K. E. 《ICES Journal of Marine Science》1999,56(5):658-672

  相似文献   

淡水珍珠中文石球粒的发现与成珠机制探讨   总被引：2，自引：1，他引：1  

张妮  郭继春  张学云  李加贵 《矿物学报》2005,25(3):307-311

长期以来,珍珠的成因理论很少涉及在超显微镜结构研究基础上的具体的珍珠形成机制。珍珠与贝壳珍珠层具有较多的相似性,但也存在很多不同。本文在对AFM下首次观察到的淡水珍珠表面纳米级的六方最紧密堆积文石球粒的研究基础上,结合XRD分析对珍珠内部矿物成分差异的研究,试图在贝壳珍珠层成因理论的基础上探讨淡水珍珠的成珠机制。本文将珍珠的形成过程初步归纳为碳酸钙凝胶的形成,碳酸钙球粒的形成,矿物相的转变和后继生长四个阶段。  相似文献   

三种碳源对乌梁素海好氧不产氧光合细菌群落结构的影响   总被引：3，自引：1，他引：2  

赵吉睿  巩瑞红  李畅游  王瑞刚  陈晨  陈勿力吉玛  牛晓峰  冯福应 《湖泊科学》2014,26(1):113-120

好氧不产氧光合细菌(AAPB)营光合异养,在水体碳循环中起着重要作用.但是,碳源对AAPB群落结构的影响尚不明确.本文基于16S rDNA和光合反应中心合成中的关键基因——pufM基因,运用变性梯度凝胶电泳(DGGE)技术,比较分析了在微生物分离培养中常用的3种碳源(葡萄糖、丙酮酸钠、酵母提取物)对乌梁素海水体总细菌及AAPB群落结构的影响.结果表明,不同碳源诱导7~21 d后,水体总细菌群落丰富度及多样性指数均有不同程度的提升,AAPB群落则呈现先升后降的趋势.3种碳源中,丙酮酸钠添加后对总细菌及AAPB菌群的丰富度和多样性的提升效果最好.测序及系统发育结果表明,乌梁素海水体经诱导后新检测到了一些原环境中未发现的菌属,一些具有特殊碳代谢能力的菌属丰度得到了提升.本研究为充分认识和理解AAPB的生态学作用和意义,及其富集、分离和培养提供了基础依据.  相似文献   

延迟交联凝胶研制及其在广西某铀矿堵漏应用          下载免费PDF全文

熊正强  陶士先  刘俊辉  李晓东  薛倩冰 《探矿工程》2020,47(4):140-144

针对地质钻探小井眼条件下失返性漏失堵漏技术难题,开展了适用于地质钻探堵漏要求的聚合物交联凝胶研究。采用单因素优选法,优选了交联凝胶体系用各种处理剂,并以环保型植物胶GJ为成胶剂,加入交联控制剂N1及交联剂1号等组分,研制了一种延迟交联凝胶。该凝胶在广西某铀矿勘查ZK6-37-1孔堵漏成功应用,解决了2个孔段失返性漏失问题。研究结果表明,该凝胶具有交联时间可控(交联时间10～75 min)以及较高的凝胶强度（承压≥6 MPa）,在解决失返性漏失方面具有广阔的应用前景。  相似文献   

秦岭造山带多金属矿普查复杂地层护壁堵漏技术          下载免费PDF全文

柳硕林  韩明耀 《探矿工程》2020,47(1):43-47

秦岭造山带多金属矿普查项目钻探施工过程中钻遇多种复杂地层,垮塌、缩径、漏失等复杂情况频发,严重影响了施工效率。分别采用了植物胶钙处理钻井液、聚乙烯醇无固相钻井液等进行护壁工作,结合不同漏失情况分别实施了随钻堵漏、凝胶堵漏等技术措施,取得了良好的使用效果。  相似文献   

蔚县矿区地面沉陷InSAR多维形变监测     

杜建涛  闫丽  赵超英 《煤田地质与勘探》2020,48(1):168-173

河北蔚县是我国华北地区最大的地下采煤区之一,该地区长期存在采矿塌陷灾害,不仅威胁采矿安全,而且严重破坏当地生态环境。本文基于合成孔径雷达干涉（InSAR）这一新型空间对地观测技术,采用61景Sentinel-1A/B干涉宽幅（Interferometric Wide swath,IW）模式数据进行矿区形变观测,获取整个矿区在2017—2018年间的地表形变空间分布特征,并对矿区地表的沉降量级及面积进行详细的统计分析。此外,采用融合多轨道SAR数据的多维形变时序估计方法,对西细庄矿数据进行东西向和垂向的二维形变分解,获取该矿的二维形变时间序列。结果表明:除南留庄井田外,其余三大井田在监测期间均存在不同程度的地面沉陷灾害;整个矿区年沉陷速率超过-10 cm/a的区域达到了2.16 km2;受成像几何影响,不同轨道数据获取的形变结果存在一定差异;西细庄矿以垂向形变为主,伴随明显的东西向水平形变。研究结果为蔚县矿区地面沉陷监测与煤矿安全开采提供数据参考。   相似文献   

Interaction of Freshly Precipitated Silica Gel with Aqueous Silicic Acid Solutions under Ambient and Near Neutral pH-conditions: A Detailed Analysis of Linear Rate Law     

Jiří Faimon  Martin Blecha 《Aquatic Geochemistry》2008,14(1):1-40

Interaction of freshly precipitated silica gel with aqueous solutions was studied at laboratory batch experiments under ambient and near neutral pH-conditions. The overall process showed excellent reversibility: gel growth could be considered as an opposite process to dissolution and a linear rate law could be applied to experimental data. Depending on the used rate law form, the resulting rate constants were sensitive to errors in parameters/variables such as gel surface area, equilibrium constants, Si-fluxes, and reaction quotients. The application of an Integrated Exponential Model appeared to be the best approach for dissolution data evaluation. It yielded the rate constants k _dissol ∼ (4.50 ± 0.68) × 10⁻¹² and k _growth ∼ (2.58 ± 0.39) × 10⁻⁹ mol m⁻² s⁻¹ for zero ionic strength. In contrast, a Differential Model gave best results for growth data modeling. It yielded the rate constants k _dissol ∼ (1.14 ± 0.44) × 10⁻¹¹ and k _growth ∼ (6.08 ± 2.37) × 10⁻⁹ mol m⁻² s⁻¹ for higher ionic strength (I ∼ 0.04 to 0.11 mol L⁻¹). The found silica gel solubility at zero ionic strength was somewhat lower than the generally accepted value. Based on the and standard Gibbs free energy of silica gel formation was calculated as and −850,318 ± 20 J mol⁻¹, respectively. Activation energies for silica gel dissolution and growth were determined as and respectively. An universal value for growth of any silica polymorph, is not consistent with the value for silica gel growth, which questions the hypothesis about one unique activated complex controlling the silica polymorph growth.  相似文献   

Comparative proteome analysis of butachlor-degrading bacteria   总被引：1，自引：0，他引：1  

Jinhua Wang  Yitong Lu  Yingying Chen 《Environmental Geology》2008,53(6):1339-1344

A Pseudomonas putida strain, named ER1, was isolated from an agricultural soil and found to actively degrade the herbicide butachlor. The enzyme extracted from ER1 could degrade butachlor. Furthermore, incubation of ER1 in a medium containing 50 mg/kg of butachlor after 3 days resulted in the high butachlor-degrading enzyme activity of ER1. Response of ER1 to butachlor might be related to changes in protein composition at both quantitative and qualitative levels. Total proteins were extracted from control strain (incubated in the medium without butachlor) and the treated strain (incubated in the medium with butachlor). The proteins were separated by two-dimensional gel electrophoresis. Of the total number of ER1 protein, 11 spots were significantly changed under butachlor stress. Analysis by matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry coupled with database searching allowed the function of some proteins which were similar to the hydrolases activity or oxidoreductase activity.  相似文献